Journal: Cardiology Research

Article Title: Nuclear Respiratory Factor-1 Ameliorates Heart Failure by Suppressing Cardiomyocyte Pyroptosis-Associated Signaling Via the Downregulation of Gasdermin D and Caspase-1

doi: 10.14740/cr2153

Figure Lengend Snippet: NRF-1 is negatively correlated with pyroptosis in HF patients. (a) Representative images of echocardiography in HF patients and NF controls. (b–g) Serum NT-proBNP, IL-18, IL-1β, NRF-1, GSDMD and caspase-1 level in HF patients (n = 15) and NF controls (n = 10). *P < 0.05, **P < 0.01, ***P < 0.001. GSDMD: gasdermin D; HF: heart failure; IL: interleukin; NF: normal cardiac function; NRF-1: nuclear respiratory factor-1; NT-proBNP: N-terminal pro-B-type natriuretic peptide.

Article Snippet: ELISA kits were used to detect N-terminal pro-B-type natriuretic peptide (NT-proBNP, EK1393, Multi Science, Hangzhou, China), NRF-1 (E15022h, EIAab, Wuhan, China), IL-18 (EK118S, Multi Science, Hangzhou, China), IL-1β (EK101B, Multi Science, Hangzhou, China), gasdermin D (GSDMD) (E15480h, EIAab, Wuhan, China), and caspase-1 (E1592h, EIAab, Wuhan, China), following the instructions of manufacturers.

Techniques:

Journal: Translational Psychiatry

Article Title: Astrocytic APOE3-Christchurch expression ameliorates brain amyloid-β pathology in 5xFAD mice

doi: 10.1038/s41398-026-04002-9

Figure Lengend Snippet: ( A - C ) Aβ40 and Aβ42 levels in the soluble TBS fractions ( A ), detergent soluble (TBS-X) fractions ( B ), and insoluble neutralized formic acid (FA) fractions ( C ) of cortical brain homogenates in the 5xFAD mice were measured by ELISA at 8 months of age, normalized to protein concentrations of each fraction. ( D ) Soluble oligomeric Aβ (oAβ) levels in the TBS-X fractions of cortical brain homogenates in the 5xFAD mice were measured by ELISA at 8 months of age, normalized to protein concentrations. ( E, F ) Amounts of full-length APP in the TBS-X fractions of mouse cortical lysates were measured by western blot at 8 months of age, normalized to those of β-actin (ACTB). Data are expressed as means ± SEM (GFP: N = 12; APOE3: N = 18; APOE3Ch: N = 13; Open circles: females, closed circles: males). Injection group differences were analyzed using two-way ANOVA, with Tukey’s multiple comparisons test, adjusting for sex. *, P < 0.05; **, P < 0.01.

Article Snippet: Recombinant Aβ42 monomer (1 mg/ml) (Stressmarq, #SPR-485) was incubated at room temperature for 10 min. To dissolve the peptide, 7.5 μl of cold DMSO (Sigma Aldrich) was added, followed by 92.5 μl of cold PBS (PH 7.4) to reach a final concentration of 220 μM monomeric Aβ42.

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Injection

Journal: Translational Psychiatry

Article Title: Astrocytic APOE3-Christchurch expression ameliorates brain amyloid-β pathology in 5xFAD mice

doi: 10.1038/s41398-026-04002-9

Figure Lengend Snippet: ( A ) Isogenic iPSC-derived astrocytes (iPSC-ACs) with homozygous APOE3 (left) and APOE3Ch (right) were immunostained for GFAP (green) and S100b (red). DAPI (blue) stains nuclei. Scale bars: 100 µm. ( B ) APOE mRNA levels in the iPSC-ACs were measured by RT-qPCR, normalized to those of β-actin ( ACTB ). ( C, D ) Amounts of APOE in the conditioned medium (CM) from iPSC-ACs were measured by western blot, normalized to protein amounts of cell lysates. ( E ) Effects of the CM from iPSC-ACs on Aβ42 aggregation was assessed by western blot using 6E10 antibody. ( F - G ) Populations of Aβ fibrils (F; > 150 kDa), oligomers (G; 37–150 kDa), and monomers ( H ; < 10 kDa) were quantified. Data expressed as means ± SEM (n = 3–5 independent differentiation batches). Group differences were analyzed using two-tailed student t-test or one-way ANOVA with Tukey’s multiple comparisons test. *, P < 0.05.

Article Snippet: Recombinant Aβ42 monomer (1 mg/ml) (Stressmarq, #SPR-485) was incubated at room temperature for 10 min. To dissolve the peptide, 7.5 μl of cold DMSO (Sigma Aldrich) was added, followed by 92.5 μl of cold PBS (PH 7.4) to reach a final concentration of 220 μM monomeric Aβ42.

Techniques: Derivative Assay, Quantitative RT-PCR, Western Blot, Two Tailed Test

Journal: Frontiers in Neurology

Article Title: Phase-targeted erythropoietin derivatives for traumatic brain injury: bridging mechanisms to precision therapy

doi: 10.3389/fneur.2025.1665405

Figure Lengend Snippet: Schematic overview of erythropoietin (EPO) and engineered derivatives in traumatic brain injury (TBI). The diagram illustrates phase-specific mechanisms by which EPO and its engineered derivatives (carbamylated EPO, asialo-EPO, darbepoetin alfa, and HBSP/cibinetide) mitigate secondary brain injury. HBSP and CEPO are βcR-biased ligands that preferentially activate cytoprotective PI3K–AKT and NF-κB pathways, whereas darbepoetin alfa retains STAT5 signaling associated with erythropoiesis. Color coding: orange = JAK2/STAT5; blue = PI3K/AKT; teal = MAPK/ERK; purple = INPP5D/NF-κB.

Article Snippet: Peptide truncation and mimetics : Efforts to minimize molecule size while retaining function have led to the development of helix-B surface peptide (HBSP) and EPObis, short synthetic peptides derived from the EPO binding interface ( , ).

Techniques:

Journal: Frontiers in Cardiovascular Medicine

Article Title: A swine model of severe chronic thromboembolic pulmonary hypertension induced by repeated pulmonary artery long suture injection

doi: 10.3389/fcvm.2025.1736958

Figure Lengend Snippet: Right ventricular myocardium gene expression (A) and protein levels (B) , and circulating plasma mediator levels (C) in chronic thromboembolic pulmonary hypertension (CTEPH) and sham (sham). Gene expression data depicts genes involved in myofilament remodeling (MYH6 and MYH7, α- and β-myosin heavy chain isoforms, respectively), extracellular matrix fibrosis (COL1A1 and COL3A1, type I and III collagen chains, respectively), Ca 2+ -handling (ATP2A2, sarcoendoplasmic reticulum calcium ATPase 2; RYR2, cardiac ryanodine receptor), and neurohumoral mediators (NPPB, B-type natriuretic peptide; EDN1, endothelin-1; TNF, tumor necrosis factor-α). Data were averaged upon normalization for 2 internal control genes, GAPDH (glyceraldehyde-3-phosphate dehydrogenase) and RPL4 (ribosomal protein L4). Calcium-handling protein levels of sarcoendoplasmic reticulum Ca2+-ATPase 2a (SERCA2a) normalized for GAPDH and phospho-phospholamban (PLB) normalized for total PLB and representative Western blot bands are presented in (B) , whilst mediator plasma levels of N-terminal pro–B-type natriuretic peptide (NT-proBNP) and endothelin-1 (ET-1) are presented in panel C. In panels A and B data are presented relative to Sham reference levels (dashed lines). * P < 0.05 vs. Sham by Student's t -test or Mann–Whitney U -test, according to assumptions ( n = 6 and 7 in Sham and CTEPH, respectively).

Article Snippet: Previously aliquoted plasma samples were thawed on ice and assayed for N-terminal pro–B-type natriuretic peptide (NT-proBNP; CSB-EQ027465PI, Cusabio) and endothelin-1 (ET-1; ADI-900-020A, Enzo Life Sciences Inc) in duplicate according to the manufacturer's instructions.

Techniques: Gene Expression, Clinical Proteomics, Control, Western Blot, MANN-WHITNEY